Methodologies and Computation - Contents

The first step

Databases

Purpose of databases

Example of a database: GenBank

Finding databases

Using databases

Databases are dynamic and variable

Tools for using databases

Phred and Phrap

FastA

Sequence retrieval

Sequence alignment

ESTs

EST production pictorial

Use of ESTs

Available ESTs

Limitations of ESTs

Finding genes

Finding genes using gene-finding programs

Finding genes using other known genes

Other methods of finding genes

Gene function

Identifying the function of a gene

The study of gene expression

Gene expression analysis

Microarrays

Use of microarrays

Limitations of microarrays

Resources & additional information