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Methodologies and Computation
Methodologies and Computation - Contents
The first step
Databases
Purpose of databases
Example of a database: GenBank
Finding databases
Using databases
Databases are dynamic and variable
Tools for using databases
Phred and Phrap
FastA
Sequence retrieval
Sequence alignment
BLAST
How BLAST works
How BLAST works - pictorial
Interpreting BLAST output
BLAST parameters
The BLAST suite
Large scale computational genomics
BAC end sequencing and methyl-filtration libraries
Use of C0t analysis
ESTs
EST production pictorial
Use of ESTs
Available ESTs
Limitations of ESTs
Finding genes
Gene function
The study of gene expression
Gene expression analysis
Microarrays
Use of microarrays
Limitations of microarrays
Resources & additional information
Sections:
About Genomics
Basic Concepts
Methodologies and Computation
Applications and Discoveries
Resources
How BLAST works - pictorial