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Prerequisites for QTL mapping

•Availability of a good linkage map (this can be done at the same time the QTL mapping)
•A segregating population derived from parents that differ for the trait(s) of interest, and which allow for replication of each segregant, so that phenotype can be measured with precision (such as RILs or DHs)
•A good assay for the trait(s) of interest
•Software available for analyses