Considerations in MAB
However, the specifics of these steps must be tailored to each project. Some things to be decided include:
- Type of marker to be used
- What type and which generation of population(s) will be developed
- At which stage or generations of the process markers should be applied
- Number of markers to be used
- Number of plants per population/generation
- Experimental design
- Type of analyses needed
These will depend on the desired result of the project (an improved breeding line, hybrid, or open-pollinated variety?) and the particular limitations of the crop (level of genetic diversity, fertility, propagation system, etc.)