QTL discovery in barley (1)
A good example of QTL discovery can be seen in von Korff et al. (2008). A wild relative of barley was crossed to a spring barley cultivar, and an advanced backcross population (BC2DH) was generated.
•Problem to address: Malting quality is determined by the complex interaction of numerous traits.
•Goal: To identify QTL in exotic germplasm for improving malting quality traits.
•Method: The advanced-backcross strategy (Tanksley and Nelson 1996) was used.
•Germplasm: a BC2DH population of 301 lines was developed from a cross between a spring barley cultivar and a wild barley accession.
Von Korff M, Wang H, Leon J, Pillen K (2008) AB-QTL analysis in spring barley: III. Identification of exotic alleles for the improvement of malting quality in spring barley (H. vulgare ssp. spontaneum). Mol Breeding 21: 81-93