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Extraction of DNA

Obtaining DNA of sufficient quality and quantity is an important consideration for MAB. Some markers need relatively large quantities of high grade DNA, others work well with small amounts of low grade DNA. The required quality and quantity of DNA need to be considered when deciding which markers, and which DNA extraction protocol to use.

For example, AFLPs require about higher amounts of high quality DNA, but an SSR assay works with 10ng of lesser quality DNA (more on types of markers later).

A laboratory technician carefully adds chloroform to a large DNA extraction prep.

For DNA extraction protocols and troubleshooting, see the Resource Center & Helpdesk