Untitled Document


If a RAPD or an AFLP fragment appears to be correlated with the presence of a favourable allele at an important gene, it can be converted to a more reliable marker called a Sequence Characterized Amplified Region (Paran and Michelmore 1993) (or simply a Sequence-tagged site, STS).

The idea is to first purify the DNA fragment (by cutting it out of the gel), then clone it. The DNA sequence of the clone will allow for specific primers to be designed. Obviously this is not doable on a large scale, but it is a useful way of exploiting individual RAPD or AFLP fragments in MAB.