Sections:

Marker-assisted Breeding

Selection of Markers

Genetic Diversity

Tips for Phenotyping

Genetic Linkage Mapping

Note on terminology

What are genetic maps?

The Punnett square

Mendel’s law of independent assortment

Frequency of recombination

Using recombination for genetic mapping

Observing recombination

Observing recombination frequencies

Turning RF into map distances

Double crossovers

Interference in recombination

Making genetic maps

Using markers to assess recombination

Using markers to assess recombination

Marker example, contd.

Marker scoring for mapping

Use of LOD scores

Mapping Software: what it can do for you

The end of genetic maps

Example: tomato

From linkage group to chromosome

Genetic distance vs. physical distance

Comparative mapping

Available software programs

Quantitative Trait Analysis

Applications & Future Directions

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Using markers to assess recombination

This figure shows the profile of parents 1 and 2 assayed with CAPs marker X. The first lane is a size standard. There is a clear polymorphism between the two parents. If both parents showed the same band (i.e. they were monomorphic for this marker), this would not be a useful marker.

Note: CAPS markers identify polymorphisms by digesting the PCR products with a restriction enzyme. The parent 2 profile consists of two restriction fragments, but the parent 1 amplified segment contains no restriction site, and thus is not cut by this particular enzyme.