Untitled Document

Mapping data

The partial data file below shows allele calls for 83 F2 progeny at five loci.

It is important that the scores for each plant are in exactly the same order for each marker so that recombinations can be identified.

Note the presence of scores that are 4 or 5 – what are these? Some mapping programs allow for ambiguity. For example, there may be a band pattern on your gel that you are sure is not a "3", but can’t decide whether it is a "1" or a "2". In this situation you might score this as a "5" (and a pattern that is either a "2" or a "3" might be scored as "4"). This is highly dependent on the software program you use. In this case missing data is scored as “0”.