The Molecular Breeding Design Tool is a graphical application that can be used to manage a marker-assisted back-crossing project to introgress QTL from a donor into a recipient germplasm. The MBDT is highly graphical, facilitating the comparison of germplasm based on a particular genotypic profile. The tool can be used to design ideotypes based on QTL target regions (foreground markers) and to recover recurrent parent genome (background markers) in back-cross breeding applications.
The graphical display facilitates the comparison of germplasm based on genotype; select donor and recurrent parents; define a target genotype (ideotype); assign foreground markers associated with QTL; assign background markers to recover recurrent parent genome; and, plan crosses and monitor progress through back-cross cycles.
MBDT includes additional tools to determine the minimum number of individuals that need to be genotyped in each BC generation to recover at least one double homozygote for the markers flanking target QTL at the end of the programme; and, identify polymorphic makers between any two accessions.
Whereas MBDT is accessible as part of the integrated tools of the IBP Breeding Management System, it can also be downloaded and used as an independent standalone tool. A tutorial for MBDT is available in our training section.