KASP Markers for Cassava
The genetic map was taken from the work of Rabbi et al. (2012). A genome-wide SNP-based genetic map of cassava anchored in SSRs was created. An outbreeder full-sib (F1) was developed by crossing Namikonga (female) and Albert (male). The F1 was genotyped on two independent SNP assay platforms: an array of 1536 SNPs on Illumin’s GoldenGate platform was used to genotype a first batch of 60 F1. Of the 1358 successfully converted SNPs, 600 which were polymorphic in at least one of the parents and was subsequently converted to KBiosciences KASPar assay platform for genotyping 70 additional F1. High-precision genotyping of 163 informative SSRs using capillary electrophoresis was also carried out. Linkage analysis resulted in a final linkage map of 1837 centi-Morgans (cM) containing 568 markers (434 SNPs and 134 SSRs) distributed across 19 linkage groups (Rabbi, 2012).
Rabbi, IY, HP Kulembeka, E Masumba, PR Marri, M Ferguson. 2012. An EST-derived SNP and SSR genetic linkage map of cassava (Manihot esculenta Crantz). Theor Appl Genet. 125: 329 – 342.KASP MARKERS FOR GENOTYPINNG